This research proposal examines the complex photophysical behavior of GFP and its chromophore analogues, focusing on the interplay between bright fluorescent states and dark non-emissive states. It aims to characterize the formation and decay of dark excited states in ortho- and para-GFP isomers, elucidate the role of cis-trans isomerization, and identify factors controlling radiative and non-radiative decay pathways.
Key findings
Direct observation and characterization of dark excited states in GFP isomers.
Elucidation of cis-trans isomerization's role in photophysics of each isomer.
Identification of structural and electronic factors controlling decay pathways.
Limitations & open questions
Further research needed to fully understand the protein-chromophore interactions.
The complexity of excited-state processes may require additional experimental techniques.