This research proposes a multimodal platform integrating Stimulated Raman Photothermal Scattering (SRPSCAT) with interferometric scattering (iSCAT) mass photometry for comprehensive single-molecule protein characterization without the need for extrinsic labels.
Key findings
Simultaneous acquisition of chemical fingerprints via Raman spectroscopy and precise mass measurements through interferometric scattering.
Correlation of vibrational spectroscopic signatures with molecular mass and scattering properties for structural, chemical, and physical information.
Development of an integrated optical instrument for SRPSCAT and iSCAT measurements and advanced correlative analysis algorithms.
Limitations & open questions
The research is in the proposal stage and has not yet been conducted, thus no experimental limitations are discussed.